Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Modification of Disp3 expression levels by CRISPR/Cas9 technology affects neural stem cells capacity to differentiate.

ABSTRACT: DISP3 (PTCHD2), a sterol-sensing domain-containing protein, is highly expressed in neural tissue but its role in neural differentiation is unknown. To determine the effect of DISP3 on neural stem cells phenotype of NS-5 cells, we impaired Disp3 gene using CRISPR/Cas9 technology. Modification of Disp3 expression levels affects NS-5 capacity to differentiate. In differentiated Disp3 sgRNAs cell cultures we observed higher number of highly differentiated astrocytes and oligodendrocytes and neurons with not only increased Î²III-tubulin mRNA expression but also with more developed neurites.

ORGANISM(S): Mus musculus

SUBMITTER: Michal Kolar

PROVIDER: E-MTAB-4485 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Modulated DISP3/PTCHD2 expression influences neural stem cell fate decisions.

Konířová Jana J Oltová Jana J Corlett Alicia A Kopycińska Justyna J Kolář Michal M Bartůněk Petr P Zíková Martina M

Scientific reports 20170130

Neural stem cells (NSCs) are defined by their dual ability to self-renew through mitotic cell division or differentiate into the varied neural cell types of the CNS. DISP3/PTCHD2 is a sterol-sensing domain-containing protein, highly expressed in neural tissues, whose expression is regulated by thyroid hormone. In the present study, we used a mouse NSC line to investigate what effect DISP3 may have on the self-renewal and/or differentiation potential of the cells. We demonstrated that NSC differe ...[more]

PMID: 28134287

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Dataset Information

Modification of Disp3 expression levels by CRISPR/Cas9 technology affects neural stem cells capacity to differentiate.

Publications

Modulated DISP3/PTCHD2 expression influences neural stem cell fate decisions.

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