Defining Immune Heterogeneity in HNSCC with RNAseq

ABSTRACT: We collected up to 6 separate analytes per patient from 14 HNSCC individuals and evaluated spatial (9 cases) and/or temporal (8 cases) variability using RNAseq. Sections taken from a frozen section using a crytostat were analysed for RNA quality was assessed using the Agilent 2100 Bioanalyser (Agilent Technologies UK Ltd., Stockport, UK). Total RNA was converted into a library for sequencing on the HiSeq 2000 (Illumina Inc., San Diego, USA) using the TruSeq™ stranded mRNA Sample Preparation Kit (Illumina Inc.). Briefly, poly-A mRNA was purified from total RNA (100ng) using the Poly(A) Purist Mag Kit (Life Technologies Ltd., Paisley, UK), according to the manufacturer’s instructions. The mRNA was then amplified and converted into cDNA, which was purified and used to construct libraries

INSTRUMENT(S): Illumina HiSeq 2000

ORGANISM(S): Homo sapiens

SUBMITTER: James Clarke 

PROVIDER: E-MTAB-4546 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress