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RNA-seq of non-ischemic heart tissues treated with a traditional Chinese medicine formula (BYD) against myocardial ischemia in Sprague Dawley rats

ABSTRACT: The myocardial tissues of the ischemic left ventricle and the peri-ischemic rim (an approximately 1 mm rim of normal-appearing tissues) were removed, and the remaining tissues that consisted of non-ischemic left ventricle were obtained for the transcriptional analysis. The total RNA was extracted from the myocardia from the sham (n = 3), myocardial ischemia model (n = 3), and a traditional Chinese medicine formula treated on myocardial ischemia (n = 3) groups. Beads that contained oligo were used to isolate the poly mRNA from the total RNA and then the rRNA was subsequently removed. The amplified library was sequenced on a HiSeq 2500 sequencing machine (Illumina, San Diego, California, USA) with a 50 bp SE strategy. Using CASAVA software, the images generated by the Illumina sequencers were converted into raw reads by base-calling.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Rattus norvegicus

SUBMITTER: Zhiyong Du

PROVIDER: E-MTAB-6509 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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