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Chromatin immunopreciptiation to determine the genome wide locatio of different components of the RNA polymerase II preinitiation complex in yeast

ABSTRACT: Genome wide location of different components of RNA polymerase II preinitiation complex in yeast S.cerevisiae

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Yad Ghavi-helm

PROVIDER: E-MEXP-1502 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Mediator-dependent recruitment of TFIIH modules in preinitiation complex.

Esnault Cyril C Ghavi-Helm Yad Y Brun Sylvain S Soutourina Julie J Van Berkum Nynke N Boschiero Claire C Holstege Frank F Werner Michel M

Molecular cell 20080801 3

In vitro, without Mediator, the association of general transcription factors (GTF) and RNA polymerase II (Pol II) in preinitiation complexes (PIC) occurs in an orderly fashion. In this work, we explore the in vivo function of Mediator in GTF recruitment to PIC. A direct interaction between Med11 Mediator head subunit and Rad3 TFIIH subunit was identified. We explored the significance of this interaction and those of Med11 with head module subunits Med17 and Med22 and found that impairing these i ...[more]

PMID: 18691966

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