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Transcriptomic profiling of antigen-specific T cells at the single-cell level after clearance of hepatitis C virus

ABSTRACT: We used scRNA-seq data generated using Smart-seq2 to reconstruct the native TCR from Ag-specific T cells and then to link these with the gene expression profile of individual cells. antigen specific CD8 T cells were obtained from a subject who had previously cleared hepatitis C virus (HCV) infection. A cell line was also generated from the same subject and included in the analysis with and without antigen stimulation.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Fabio Luciani

PROVIDER: E-MTAB-4850 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Linking the T cell receptor to the single cell transcriptome in antigen-specific human T cells.

Eltahla Auda A AA Rizzetto Simone S Pirozyan Mehdi R MR Betz-Stablein Brigid D BD Venturi Vanessa V Kedzierska Katherine K Lloyd Andrew R AR Bull Rowena A RA Luciani Fabio F

Immunology and cell biology 20160210 6

Heterogeneity of T cells is a hallmark of a successful adaptive immune response, harnessing the vast diversity of antigen-specific T cells into a coordinated evolution of effector and memory outcomes. The T cell receptor (TCR) repertoire is highly diverse to account for the highly heterogeneous antigenic world. During the response to a virus multiple individual clones of antigen specific CD8+ (Ag-specific) T cells can be identified against a single epitope and multiple epitopes are recognised. A ...[more]

PMID: 26860370

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