Transcriptional profiling of wild-type parent MC58 strain and ptsH knock-out mutant in Neisseria meningitidis

ABSTRACT: In order to assess global differences in gene expression that may be regulated directly or indirectly by HPr , a global transcriptomic profile analysis of the ΔptsH mutant and the wild-type MC58 strain was performed. cDNA probes were prepared from RNA pools (5 μg) obtained from MC58 wild-type and ΔptsH mutant cells grown in rich GCB broth until mid-logarithmic phase. RNA was extracted from mid-log phase and differential gene expression through microarray analysis was performed. Equal amounts of Cy5- and Cy3-labelled cDNAs were hybridized onto the microarray for 17 h at 65°C according to the Agilent protocol. Three hybridizations were performed using cDNA probes from three independent pools, respectively, corresponding to RNA extracted from a total of nine independent bacterial cultures for each strain. Images were acquired by using an Agilent microarray scanner G2505B at 5 µm of resolution and using the extended dynamic range and were analysed with Agilent Feature Extraction 9.5.1. Differentially expressed genes were assessed by grouping all log2 ratios of the Cy5 and Cy3 values corresponding to each gene, within experimental replicas and spot replicas, and comparing them against the zero value by Student's t test statistics.

ORGANISM(S): Neisseria meningitidis MC58

SUBMITTER: Isabel Delany 

PROVIDER: E-MTAB-4997 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress