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Insights into the prevalence and underlying causes of clonal variation through transcriptomic analysis in Pichia pastoris

ABSTRACT: Clonal variation, wherein a range of specific productivities of secreted proteins are observed from supposedly identical transformants, is an ingrained aspect of working with Pichia pastoris. It means that a significant number of transformants need to be tested to obtain a representative sample, and in commercial protein production, companies regularly screen thousands of transformants to select for the highest secretor. Here we have undertaken a detailed investigation of this phenomenon by characterising clones transformed with the human serum albumin gene. In order to evaluate the prevalence and underlying causes of clonal variation, nine strains were selected, each transformed with a single copy of the human serum albumin (HSA) gene. All strains were subjected to a wide-ranging evaluation to understand the implications of this phenomenon.

ORGANISM(S): Komagataella pastoris

SUBMITTER: Geraint Barton

PROVIDER: E-MTAB-5124 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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