Dataset Information

Global quantification of intact proteins via chemical isotope labeling and mass spectrometry

ABSTRACT: Although thousands of intact proteins can be feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation efficiency is as high as 99.2% for complex intact protein samples extracted from HeLa cells. Further, the pTop 2.0 software is developed for automated quantification of intact proteoforms in a high-throughput manner. The high quantification accuracy and reproducibility of this strategy have been demonstrated for both standard and complex cellular protein samples. A total of 2,283 intact proteoforms originated from 660 protein accessions are successfully quantified under anaerobic and aerobic conditions and the differentially expressed proteins are observed to be involved into the important biological processes such as stress response.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Saccharomyces Cerevisiae (baker's Yeast)

SUBMITTER: Zheyi Liu

LAB HEAD: Fangjun Wang

PROVIDER: PXD012526 | Pride | 2019-04-30

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	20160306_YEAST_controlD_HD_A1_1.raw	Raw
	20160306_YEAST_controlD_HD_A1_2.raw	Raw
	20160306_YEAST_controlD_HD_A1_3.raw	Raw
	20160306_YEAST_controlD_HD_A2_1.raw	Raw
	20160306_YEAST_controlD_HD_A2_2.raw	Raw

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Publications

Global Quantification of Intact Proteins via Chemical Isotope Labeling and Mass Spectrometry.

Liu Zheyi Z Wang Ruimin R Liu Jing J Sun Ruixiang R Wang Fangjun F

Journal of proteome research 20190419 5

Although thousands of intact proteins have been feasibly identified in recent years, global quantification of intact proteins is still challenging. Herein, we develop a high-throughput strategy for global intact protein quantification based on chemical isotope labeling. The isotope incorporation efficiency is as high as 99.2% for complex intact protein samples extracted from HeLa cells. Further, the pTop 2.0 software is developed for automated quantification of intact proteoforms in a high-throu ...[more]

PMID: 30990045

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