Browse
Submit Data
Databases
API
Help

Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

65 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcription and translation profiling of rat cardiomyocytes treated with insulin

ABSTRACT: Effect of insulin on the cardiomyocyte global transcriptome and RNAs recruited to polysomes.
For comparison with endothelin-1

ORGANISM(S): Rattus norvegicus

SUBMITTER: Angela Clerk

PROVIDER: E-MEXP-2527 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

ACCESS DATA

Json Xml

Publications

Regulation of the cardiomyocyte transcriptome vs translatome by endothelin-1 and insulin: translational regulation of 5' terminal oligopyrimidine tract (TOP) mRNAs by insulin.

Markou Thomais T Marshall Andrew K AK Cullingford Timothy E TE Tham El L el L Sugden Peter H PH Clerk Angela A

BMC genomics 20100529

<h4>Background</h4>Changes in cellular phenotype result from underlying changes in mRNA transcription and translation. Endothelin-1 stimulates cardiomyocyte hypertrophy with associated changes in mRNA/protein expression and an increase in the rate of protein synthesis. Insulin also increases the rate of translation but does not promote overt cardiomyocyte hypertrophy. One mechanism of translational regulation is through 5' terminal oligopyrimidine tracts (TOPs) that, in response to growth stimul ...[more]

PMID: 20509958

Similar Datasets

BIGenomics_bioreactor_samples

Project description:The aim of the study was to use microarray for profiling the microbiota in anaerobic digestion process. The probes are ssDNA molecules that are ligated into circular molecules if a complementary target sequence is present in the sample DNA. Ligated probes are PCR amplified with a labeled primer, and the amplicons are hybridized on DNA microarray by tag sequences.

2012-02-06 | E-MEXP-3541 | biostudies-arrayexpress

Translational profiling of HEK293 cells exposed to cold shock (32 degrees C) and untreated (37 degrees C)

Project description:This experiment investigates the effect of mild cold-shock (32 degrees C, 24 hours) on the translatome of human HEK293 cells. After cold-shock, cells were treated with cycloheximide to block elongation, then sucrose density gradients were used to separate post-nuclear extracts into polysome and subpolysome fractions. The pooled polysomal fraction was labelled with Cy5, the pooled subpolysomal fraction was labelled with Cy3, and both were hybridised to the same array.

2017-02-23 | E-MTAB-5437 | biostudies-arrayexpress

Transcription profiling of Synechococcus elongatus wild type, K10 and MuD mutants growth with or without Fe

Project description:Synechococcus elongatus PCC7942 wild type, the IdiB-free S.elongatus mutant K10 (Michel et al. 1999; Microbiology, 145: page 1473-1484) and the IdiC-merodiploid mutant MuD (Pietsch et al. 2007, Photosynth. Res. 94: page 91-108) were cultivated in BG11 medium, continuously bubbled with 2%CO2-enriched air and illuminated with fluorescent bulbs with a light intensity of 100 ﾵmol photons m-s s-1. After an inoculation of a cell density of OD750nm 0.3 the cells were cultivated with iron-sufficient or iron-deficient BG11 medium. The growth time for wild type was 24h and 72h. The two mutants were cultivated for 72h only. Possible differences in the RNA amount after the cultivation with or without iron were examined using the microarray technique. .

2008-07-13 | E-MEXP-1657 | biostudies-arrayexpress

Expression data from polysomal, nonpolysomal, and total RNAs in the S. cerevisiae tRNA trafficking mutants, msn5 and mtr10

Project description:tRNAs are transcribed and partially processed in the nucleus before they are exported to the cytoplasm where they have an essential role in protein synthesis. Surprisingly, mature cytoplasmic tRNAs shuttle between nucleus and cytoplasm and its distribution is nutrient-dependent. At least three members of M-NM-2-importin family, Los1, Mtr10, and Msn5, function in tRNA nuclear-cytoplasmic intracellular movement. To test the hypothesis that the tRNA retrograde pathway regulates translation of particular transcripts We compared individual translation activity index (P/NP), obtained from the ratio of polysome-associated (P) to not associated (non-polysomal, NP) mRNAs, in the msn5M-NM-^T, mtr10M-NM-^T, and wild-type cells under fed or acute amino acid depletion conditions Polysome associated (P), not associated (non-polysomal, NP), and total (T) RNAs were isolated from yeast cells, including wild-type (BY4742), msn5 deletion, mtr10 deletion, grown in fed and 30-min amino acid starvation conditions

2013-09-01 | E-GEOD-47713 | biostudies-arrayexpress

Transcriptional profiling of Saccharomyces cerevisiae exposed to Tripelennamine in time course.

Project description:This study newly identified Tripelennamine (TA) as an inhibitor of yeast meiosis and sporulation. To examine if and how exposure of sporulating yeast cells to TA changes the meiotic transcriptional program cells were sporulated for 0, 4, and 8 hours in the presence or absence of 100 uM TA.

2010-03-31 | E-MEXP-2522 | biostudies-arrayexpress

Transcriptional control of a plant stem cell niche

Project description:We used a systems approach to identify target genes and genome wide chromatin binding preferences of the transcription factor WUSCHEL (WUS) in Arabidopsis thaliana. First, we recorded changes in the transcriptome after genetically perturbing the regulatory system of the stem cell niche at various developmental stages by loss-of-function and inducible over-expression alleles of WUS and its antagonist CLV3. Then, identified direct WUS target genes by chromatin immuno-precipitation (ChIP.

2010-06-01 | E-MEXP-2499 | biostudies-arrayexpress

RNA-seq of coding RNA (polysomal and free fraction) from human adipose tissue stem cells treated with cyclopamine, purmorphamine and DMSO

Project description:Here we used activating (purmorphamine) and blocking (cyclopamine) drugs characterized of the Hedgehog pathway in adipose tissue-derived stem cells and identified mRNAs associated polysomes or free fraction in each condition.

2018-08-24 | E-MTAB-6254 | biostudies-arrayexpress

Effect of 30 or 90 min exposure to endothelin-1 on the cardiac myocyte transcriptome

Project description:Cardiac myocytes were exposed to 100 nM endothelin-1 for 30 or 90 min to determine effects on mRNA expression using Exon arrays

2012-10-01 | E-MEXP-3393 | biostudies-arrayexpress

Effect of 60 min exposure to endothelin-1 on the cardiac myocyte transcriptome

Project description:Cardiac myocytes were unstimulated or exposed to 100 nM endothelin-1 for 60 min

2012-10-01 | E-MEXP-3394 | biostudies-arrayexpress

PIP5K mediates feed-back regulation of PIN polarity in Arabidopsis

Project description:Differentially expressed genes in response to the phytohormone auxin in wild type and a conditional auxin signalling mutant

2011-07-04 | E-MEXP-3283 | biostudies-arrayexpress