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Microarray on wild-type and mutant Hesx1 embryonic stem (ES) cells cultured in serum/LIF

ABSTRACT: Molecular profile of Hesx1-deficient and Hesx1 wild-type ES cells cultured in serum/LIF conditions was evaluated by microarray analysis

INSTRUMENT(S): Axon GenePix® 4000B laser scanner (Axon Instruments)

ORGANISM(S): Mus musculus

SUBMITTER: Sara Pozzi

PROVIDER: E-MTAB-5821 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Project description:Hesx1+/+ and Hesx1-/- ES cells were cultured in 2i/LIF conditions for 5 passages, to induce a ground pluripotency state. Subsequently, inhibitors were removed to stimulate exit from ground pluripotency, and whole transcriptomics analysis was performed 24 hours after removal.

2018-05-21 | E-MTAB-5816 | biostudies-arrayexpress

IRF-5 and IRF-8 govern the response of Human Plasmacytoid Dendritic Cells following TLR9 ligation

Project description:Synthetic oligonucleotides (ODNs) containing CpG motifs stimulate human plasmacytoid dendritic cells (pDCs) to produce type-1 interferons (IFN) and pro-inflammatory cytokines. Previous studies demonstrated that interferon regulatory factors (IRFs) played a central role in mediating CpG-induced pDC activation. This work explores the inverse effects of IRF-5 and IRF-8 on CpG dependent gene expression. Results from RNA interference and microarray studies indicate that IRF-5 up-regulates TLR9-driven gene expression whereas IRF-8 down-regulates the same genes. Several findings support the conclusion that IRF-8 inhibits TLR9 dependent gene expression by directly blocking the activity of IRF-5. First, the inhibitory activity of IRF-8 is only observed when IRF-5 is present. Second, proximity ligation analysis shows that IRF-8 and IRF-5 co-localize within the cytoplasm of resting human pDC and co-translocate to the nucleus after CpG stimulation. Taken together, these findings suggest that two transcription factors with opposing functions control TLR9 signaling in human pDCs. CAL-1 cells were transfected with siRNA targeting IRF-5 (IRF-5si) and left unstimulated (n=4, technical repeats) or stimulated with K-type CpG ODN (n=4, technical repeats). CAL-1 cells were transfected with siRNA targeting IRF-8 (IRF-8si) and left unstimulated (n=4, technical repeats) or stimulated with K-type CpG ODN (n=4, technical repeats). CAL-1 cells were transfected with control siRNA (Contsi) and left unstimulated (n=4, technical repeats) or stimulated with K-type CpG ODN (n=4, technical repeats).

2016-06-01 | E-GEOD-70429 | biostudies-arrayexpress

Suppression of prp28-1 splicing defect by prp8-tes allele

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Defining the specificity of cotranslationally acting chaperones by systematic analysis of mRNAs associated with ribosome-nascent chain complexes.

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RNA coimmunoprecipitations

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2020-01-31 | E-MTAB-2438 | biostudies-arrayexpress

A novel dominant glossy mutation causes suppression of wax biosynthesis pathway and deficiency on cuticular wax in Brassica napus

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2013-04-05 | E-GEOD-45795 | biostudies-arrayexpress

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