Project description:shock treatment<br>Differential gene expression between the alternate 2La arrangements was examined for heat shock treated and control fourth instar larvae at three discrete time points to examine temporal variation in thermal stress response. One week after egg hatching, 72 fourth instar larvae of each 2La inversion polmorphism were randomly selected from multiple pans and placed into individual 13x100 mm glass culture tubes containing 2 mL of water. Heat shock treatment was carried out by placing tube racks containing larvae into a 38?C water bath for 60 min. After heat shock, larvae were placed back into 27?C water until collection, which took place 0.25 h, 2 h, and 8 h later by pooling 24 individuals into a 1.5 mL microcentrifuge tube and snap-freezing them in liquid nitrogen. Samples were stored at -80 ?C until RNA isolation. Control groups were treated identically for each time point with the only difference being that they were not exposed to the 60 min heat shock but rather placed into 27?C water<br>
Project description:Because premating behavioral isolation typically involves the interaction between traits in males and females, we generated whole-genome expression profiles of virgin males collected at the same age and diel time as the virgin females assayed in Cassone et al. (2010). Using the same Affymetrix Anopheles/Plasmodian GeneChip platform, levels and patterns of transcript abundance were examined between lab colonies of M and S Quantitative real-time PCR (qRT-PCR) was used to examine tissue-specific expression of candidate genes, as well as to validate transcription variation using RNA samples of M and S mosquitoes collected from natural populations in Burkina Faso and Cameroon. Gas chromatography-mass spectrometry was performed to examine qualitative differences in virgin male cuticular hydrocarbon composition between forms at mid-dusk and two additional time points.
Project description:Semi-field translocations to examine whether gene expression differences between Anopheles gambiae M and S occupying discrete larval habitats are due to inherent molecular form divergence or transcriptional plasticity
Project description:The Anopheles gambiae midgut harbors bacteria that proliferate upon a blood feed. We used microarrays to examine the midgut gene expression response at early stages (3hours) after an artifitial meal containing heat killed bacteria. Anopheles gambiae G3 mosquitoes 5-6 day-old were fed BSA (20% in PBS with fresh 10 mM sodium bicarbonate) with or without heat killed E. coli (equivalent of 2.5 ml of 0.8 OD) . Three pools of 10 mosquito midguts were dissected after 3h and processed for microarray analysis of gene expression.
Project description:The transcriptional profile of four tissues for the multi insecticide Anopheles gambiae (Tiassale) and lab susceptible Anopheles gambiae strain N'Gousso. The malpighian tubules, abodmen integument (containing the fat body epidermal, neuronal, muscle and oenocyte cells), midgut and remaining structures were dissected and compared two ways: (i) each body part against the corresponding whole organism (ii) resistant against corresponding susceptible body parts.
Project description:Resistant Tiassale (Ivory Coast) strain compared to the lab susceptible N'Gousso. Tiassale was exposed to deltamethrin and the survivors were left for 48 hours prior to the array being performed.
Project description:Resistant M'Be (Ivory Coast) strain compared to the lab susceptible N'Gousso. M'Be was exposed to deltamethrin and the survivors were left for 48 hours prior to the array being performed.
Project description:Resistant Bouake (Ivory Coast) strain compared to the lab susceptible N'Gousso. Bouake was exposed to deltamethrin and the survivors were left for 48 hours prior to the array being performed.
Project description:Transcription factor Met knockdown by dsRNA injection and compared to dsRNA-GFP injected control. Met was knocked down to determine whether it plays a role in response to traditional public health insecticides in addition to juvenile hormone homologs.