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Unknown,Transcriptomics,Genomics,Proteomics

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Bulk RNA-seq of of bone marrow derived mononuclear phagocytes

ABSTRACT: Dermal fibroblasts from mouse, rat, rabbit and pig, stimulated with LPS, with dsRNA (poly I:C), and controls.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Sus scrofa

SUBMITTER: Tzachi Hagai

PROVIDER: E-MTAB-6773 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Gene expression variability across cells and species shapes innate immunity.

Hagai Tzachi T Chen Xi X Miragaia Ricardo J RJ Rostom Raghd R Gomes Tomás T Kunowska Natalia N Henriksson Johan J Park Jong-Eun JE Proserpio Valentina V Donati Giacomo G Bossini-Castillo Lara L Vieira Braga Felipe A FA Naamati Guy G Fletcher James J Stephenson Emily E Vegh Peter P Trynka Gosia G Kondova Ivanela I Dennis Mike M Haniffa Muzlifah M Nourmohammad Armita A Lässig Michael M Teichmann Sarah A SA

Nature 20181024 7730

As the first line of defence against pathogens, cells mount an innate immune response, which varies widely from cell to cell. The response must be potent but carefully controlled to avoid self-damage. How these constraints have shaped the evolution of innate immunity remains poorly understood. Here we characterize the innate immune response's transcriptional divergence between species and variability in expression among cells. Using bulk and single-cell transcriptomics in fibroblasts and mononuc ...[more]

PMID: 30356220

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