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Single-cell RNAseq of whole B6 mouse E8.25 embryos

ABSTRACT: Gastrulation represents a pivotal point in mammalian development, when the basic body plan is established and cells are specified into one of the three germ layers. This is followed by rapid diversification into specific lineages and the appearance of the various cell types required to build each of the organs. The rich variety of cell types present at this stage has never been rigorously characterised in any mammalian organism, and thus insight into cell fate decisions and the underlying regulatory networks have been inaccessible. We have used droplet based single-cell RNA-sequencing to address this by profiling ~20000 cells from C57BL/6 E8.25 mouse embryos.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Wajid Jawaid

PROVIDER: E-MTAB-6153 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Defining murine organogenesis at single-cell resolution reveals a role for the leukotriene pathway in regulating blood progenitor formation.

Ibarra-Soria Ximena X Jawaid Wajid W Pijuan-Sala Blanca B Ladopoulos Vasileios V Scialdone Antonio A Jörg David J DJ Tyser Richard C V RCV Calero-Nieto Fernando J FJ Mulas Carla C Nichols Jennifer J Vallier Ludovic L Srinivas Shankar S Simons Benjamin D BD Göttgens Berthold B Marioni John C JC

Nature cell biology 20180108 2

During gastrulation, cell types from all three germ layers are specified and the basic body plan is established <sup>1</sup> . However, molecular analysis of this key developmental stage has been hampered by limited cell numbers and a paucity of markers. Single-cell RNA sequencing circumvents these problems, but has so far been limited to specific organ systems <sup>2</sup> . Here, we report single-cell transcriptomic characterization of >20,000 cells immediately following gastrulation at E8.25 ...[more]

PMID: 29311656

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Single-cell RNAseq of whole mouse E8.25 embryos

Project description:Gastrulation represents a pivotal point in mammalian development, when the basic body plan is established and cells are specified into one of the three germ layers. This is followed by rapid diversification into specific lineages and the appearance of the various cell types required to build each of the organs. The rich variety of cell types present at this stage has never been rigorously characterised in any mammalian organism, and thus insight into cell fate decisions and the underlying regulatory networks have been inaccessible. We have used droplet based single-cell RNA-sequencing to address this by profiling ~7000 cells from three E8.25 mouse embryos.

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