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Timecourse single-cell RNAseq of whole mouse embryos harvested between days 6.5 and 8.5 of development.

ABSTRACT: We have captured 100,000 single cells for single-cell RNAseq from whole mouse embryos during gastrulation and organogenesis, spanning days 6.5 to 8.5 of development, including embryonic and extraembryonic tissues. Cells were sampled every six hours, providing a continuous molecular characterisation of these processes. Cell libraries were prepared using the 10X Genomics Chromium platform.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Blanca Pijuan-Sala

PROVIDER: E-MTAB-6967 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A single-cell molecular map of mouse gastrulation and early organogenesis.

Pijuan-Sala Blanca B Griffiths Jonathan A JA Guibentif Carolina C Hiscock Tom W TW Jawaid Wajid W Calero-Nieto Fernando J FJ Mulas Carla C Ibarra-Soria Ximena X Tyser Richard C V RCV Ho Debbie Lee Lian DLL Reik Wolf W Srinivas Shankar S Simons Benjamin D BD Nichols Jennifer J Marioni John C JC Göttgens Berthold B

Nature 20190220 7745

Across the animal kingdom, gastrulation represents a key developmental event during which embryonic pluripotent cells diversify into lineage-specific precursors that will generate the adult organism. Here we report the transcriptional profiles of 116,312 single cells from mouse embryos collected at nine sequential time points ranging from 6.5 to 8.5 days post-fertilization. We construct a molecular map of cellular differentiation from pluripotency towards all major embryonic lineages, and explor ...[more]

PMID: 30787436

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