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Single cell RNA-Seq of the murine non-myocyte cardiac cellulome

ABSTRACT: We characterized single-cell transcriptional profiles of the cardiac non-myocyte cell pool in C57BL/6J mice. The cell preparation we sequenced consisted of metabolically active, nucleated non-myocyte cells from heart ventricles of female and male mice which were depleted of endothelial cells. The goals of this experiment included examining cellular diversity, identifying markers of understudied cell populations, exploring functional roles of different cell types, and characterizing sexual dimorphism in cardiac gene expression.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Dan Skelly

PROVIDER: E-MTAB-6173 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Single-Cell Transcriptional Profiling Reveals Cellular Diversity and Intercommunication in the Mouse Heart.

Skelly Daniel A DA Squiers Galen T GT McLellan Micheal A MA Bolisetty Mohan T MT Robson Paul P Rosenthal Nadia A NA Pinto Alexander R AR

Cell reports 20180101 3

Characterization of the cardiac cellulome, the network of cells that form the heart, is essential for understanding cardiac development and normal organ function and for formulating precise therapeutic strategies to combat heart disease. Recent studies have reshaped our understanding of cardiac cellular composition and highlighted important functional roles for non-myocyte cell types. In this study, we characterized single-cell transcriptional profiles of the murine non-myocyte cardiac cellular ...[more]

PMID: 29346760

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