Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

ChIP-seq in the cell line NALM-6 for RUNX1 and ETV6-RUNX1 to assess competition between the two proteins for DNA binding


ABSTRACT: RUNX1 and ETV6-RUNX1 possess the same DNA-binding runt domain and are therefore expected to bind to canonical RUNX motifs. As the ETV6-RUNX1 fusion arises in the context of native RUNX1 expression, and since RUNX1 is retained or amplified in B-ALL, the two proteins are likely to compete for the same target sites. To assess this, we performed RUNX1 ChIP-seq in the presence of exogenous ETV6-RUNX1 (or non DNA binding ETV6-RUNX1-R139G) and the reciprocal experiment: ETV6-RUNX1 ChIP (using a V5 tag) in the presence of exogenous RUNX1 or vector control.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Jason Wray 

PROVIDER: E-MTAB-12209 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2022-10-31 | E-MTAB-12207 | biostudies-arrayexpress
2013-11-04 | E-GEOD-50730 | biostudies-arrayexpress
2013-11-04 | E-GEOD-50733 | biostudies-arrayexpress
2011-01-10 | E-GEOD-24779 | biostudies-arrayexpress
2013-05-27 | E-GEOD-45377 | biostudies-arrayexpress
2011-01-10 | E-GEOD-24777 | biostudies-arrayexpress
2013-11-04 | E-GEOD-50736 | biostudies-arrayexpress
2018-02-08 | E-MTAB-6382 | biostudies-arrayexpress
2021-04-29 | PXD020045 | Pride
2021-04-29 | PXD020054 | Pride