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ScRNA-Seq of Phallusia mammillata from 2-cell to 16-cell stage

ABSTRACT: single cell RNA-Sequencing of Phallusia mammillata embryos from 2-cell to 16-cell stage

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Phallusia mammillata

SUBMITTER: Pierre Neveu

PROVIDER: E-MTAB-6506 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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MorphoSeq: Full Single-Cell Transcriptome Dynamics Up to Gastrulation in a Chordate.

Sladitschek Hanna L HL Fiuza Ulla-Maj UM Pavlinic Dinko D Benes Vladimir V Hufnagel Lars L Neveu Pierre A PA

Cell 20200420 4

Single-cell RNA sequencing (scRNA-seq) provides a leap forward in resolving cellular diversity and developmental trajectories but fails to comprehensively delineate the spatial organization and precise cellular makeup of individual embryos. Here, we reconstruct from scRNA-seq and light sheet imaging data a canonical digital embryo that captures the genome-wide gene expression trajectory of every single cell at every cell division in the 18 lineages up to gastrulation in the ascidian Phallusia ma ...[more]

PMID: 32315617

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