Ontology highlight
ABSTRACT:
INSTRUMENT(S): NextSeq 500
ORGANISM(S): Phallusia mammillata
SUBMITTER: Pierre Neveu
PROVIDER: E-MTAB-6508 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Sladitschek Hanna L HL Fiuza Ulla-Maj UM Pavlinic Dinko D Benes Vladimir V Hufnagel Lars L Neveu Pierre A PA
Cell 20200420 4
Single-cell RNA sequencing (scRNA-seq) provides a leap forward in resolving cellular diversity and developmental trajectories but fails to comprehensively delineate the spatial organization and precise cellular makeup of individual embryos. Here, we reconstruct from scRNA-seq and light sheet imaging data a canonical digital embryo that captures the genome-wide gene expression trajectory of every single cell at every cell division in the 18 lineages up to gastrulation in the ascidian Phallusia ma ...[more]