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Genome sequencing of Phallusia mammillata

ABSTRACT: Genome sequencing of a single Phallusia mammillata individual

INSTRUMENT(S): Illumina MiSeq

ORGANISM(S): Phallusia mammillata

SUBMITTER: Pierre Neveu 

PROVIDER: E-MTAB-6530 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

MorphoSeq: Full Single-Cell Transcriptome Dynamics Up to Gastrulation in a Chordate.

Sladitschek Hanna L HL   Fiuza Ulla-Maj UM   Pavlinic Dinko D   Benes Vladimir V   Hufnagel Lars L   Neveu Pierre A PA  

Cell 20200420 4

Single-cell RNA sequencing (scRNA-seq) provides a leap forward in resolving cellular diversity and developmental trajectories but fails to comprehensively delineate the spatial organization and precise cellular makeup of individual embryos. Here, we reconstruct from scRNA-seq and light sheet imaging data a canonical digital embryo that captures the genome-wide gene expression trajectory of every single cell at every cell division in the 18 lineages up to gastrulation in the ascidian Phallusia ma  ...[more]

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