Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Pei Wang
PROVIDER: E-GEOD-26862 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
Wang Pei P Rodriguez Ryan T RT Wang Jing J Ghodasara Amar A Kim Seung K SK
Cell stem cell 20110301 3
Human embryonic stem cells (hESCs) can provide insights into development of inaccessible human tissues such as embryonic endoderm. Progress in this area has been hindered by a lack of methods for isolating endodermal cells and tracing fates of their differentiated progeny. By using homologous recombination in human ESCs, we inserted an enhanced green fluorescent protein (eGFP) transgene into the SOX17 locus, a postulated marker of human endoderm. FACS purification and gene expression profiling c ...[more]