RNA-seq of the malaria parasite Plasmodium falciparum 3D7 during its intraerythrocytic development cycle

ABSTRACT: The parasite Plasmodium falciparum is responsible for severe malaria, which is still one of the major causes of death in developing countries. To provide a new RNA-seq reference dataset for its blood-stage transcriptome according to current guidelines and best practices, we performed a time course experiment with three independent biological replicates of synchronized P. falciparum 3D7 cells, that were cultivated at a haematocrit of 5% in human O+ erythrocytes. RNA-seq samples were taken at 8 developmental stages including young ring stage (8 hpi), late ring stage/early trophozoite (16 hpi), mid-age trophozoite (24 hpi), late trophozoite (32 hpi), early schizont (40 hpi), schizont (44 hpi), late schizont (48 hpi) and purified merozoites (0 hpi). Red blood cell pellets were lysed with Trizo

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Plasmodium falciparum 3D7

SUBMITTER: Tim Gilberger 

PROVIDER: E-MTAB-7731 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress