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Cheung SMC 2011

ABSTRACT: Transcriptional profiling of origin-specific smooth muscle cells, derived from human embryonic stem cells via chemically-defined cell culture media.

ORGANISM(S): Homo sapiens

SUBMITTER: Christine Cheung

PROVIDER: E-MTAB-781 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Generation of human vascular smooth muscle subtypes provides insight into embryological origin-dependent disease susceptibility.

Cheung Christine C Bernardo Andreia S AS Trotter Matthew W B MW Pedersen Roger A RA Sinha Sanjay S

Nature biotechnology 20120115 2

Heterogeneity of embryological origins is a hallmark of vascular smooth muscle cells (SMCs) and may influence the development of vascular disease. Differentiation of human pluripotent stem cells (hPSCs) into developmental origin-specific SMC subtypes remains elusive. Here we describe a chemically defined protocol in which hPSCs were initially induced to form neuroectoderm, lateral plate mesoderm or paraxial mesoderm. These intermediate populations were further differentiated toward SMCs (>80% MY ...[more]

PMID: 22252507

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