Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

RNA sequencing of mechanically intact and degraded murine tail tendon fascicles after exposure to differential temperature and oxygen tension

ABSTRACT: Tendon fascicles were extracted from tails of freshly euthanized mice and cultured for 6 days ex vivo in serum containing medium (10%) at either 3% oxygen and 29 degrees celsius or 21% oxygen and 37 degrees celsius.

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Jess Snedeker

PROVIDER: E-MTAB-7832 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Tendon response to matrix unloading is determined by the patho-physiological niche.

Wunderli Stefania L SL Blache Ulrich U Beretta Piccoli Agnese A Niederöst Barbara B Holenstein Claude N CN Passini Fabian S FS Silván Unai U Bundgaard Louise L Auf dem Keller Ulrich U Snedeker Jess G JG

Matrix biology : journal of the International Society for Matrix Biology 20200107

Although the molecular mechanisms behind tendon disease remain obscure, aberrant stromal matrix turnover and tissue hypervascularity are known hallmarks of advanced tendinopathy. We harness a tendon explant model to unwind complex cross-talk between the stromal and vascular tissue compartments. We identify the hypervascular tendon niche as a state-switch that gates degenerative matrix remodeling within the tissue stroma. Here pathological conditions resembling hypervascular tendon disease provok ...[more]

PMID: 31917255

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Project description:This study measured the differential expression of genes from a single clonal Daphnia pulex isolate obtained from an eutrophic flooded opencast mine near Gräfenhain (Saxony, Germany) and has been kept in the laboratory since 2002. The daphniids (50 adult animals per batch) were raised in 3-L glass beakers under a 16 h:8 h L:D photoperiod. Three-quarter of the culture medium (M4) was renewed once a week, and the animals were equally fed ad libitum with algae (Desmodesmus subspicatus; SAG 53.80, Göttingen, Germany). Long-term-acclimated animals were kept at three different temperatures (i.e., 10 ± 0.5°C, 20 ± 0.3°C, and 24 ± 0.3°C) under normoxia (Po2 ≥ 20 kPa) for at least twelve weeks. Time-resolved experiments (acute heat stress) were carried out with long-term 20°C acclimated animals, which were exposed for three different time intervals (2, 4, and 8 h) to either 30 ± 0.2°C (test conditions) or 20 ± 0.3°C (control conditions). For this, 50 animals each were rapidly transferred with a sieve (mesh size: 1.2 mm) from 20°C-medium to a set of beakers containing 30°C-medium. As control, 50 animals each were transferred from 20°C-medium to another set of beakers with 20°C-medium. Only adult female animals with a body length of 2−2.5 mm (between the base of the apical spine and the anterior part of the head) and carrying parthenogenetic eggs and embryos were used for experiments. Animals were not fed during short-term exposures. During experiments, all animals were in good physical condition. After thermal acclimation or heat exposure, animals were transferred into 1.5-ml microcentrifuge tubes and shock-frozen in liquid nitrogen after removing adhering water with a tissue paper. Samples were short-term stored at -80°C. For all experimental conditions, four independent replicates (50 animals each) were analyzed. This GEO record is for the contrasts between animals conditioned to 10 degrees Celsius (condition 1) versus 20 degrees Celsius (condition 2), 10 degrees Celsius (condition 1) versus 24 degrees Celsius (condition 3), and 20 degrees Celsius (condition 2) versus 24 degrees Celsius (condition 3)

Dataset Information

RNA sequencing of mechanically intact and degraded murine tail tendon fascicles after exposure to differential temperature and oxygen tension

Publications

Tendon response to matrix unloading is determined by the patho-physiological niche.

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