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Tissue culture model of hypoxia

ABSTRACT: Validation of an in vitro model of hypoxia using a primary cell culture of mouse embryonic fibroblasts (MEF) exposed to 1% oxygen at 37 degrees celsius. Control cells were exposed to atmospheric oxygen.

ORGANISM(S): Mus musculus

PROVIDER: GSE54228 | GEO | 2014/03/01

SECONDARY ACCESSION(S): PRJNA235990

REPOSITORIES: GEO

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Tissue culture model of hypoxia

Project description:Validation of an in vitro model of hypoxia using a primary cell culture of mouse embryonic fibroblasts (MEF) exposed to 1% oxygen at 37 degrees celsius. Control cells were exposed to atmospheric oxygen. Hypoxic and normoxic MEF (in 100 mm dishes, four replicates per group) were submitted to gene expression analysis using Affymetrix Exon microarrays. Differential gene expression analysis was performed with R package DEMI (http://biit.cs.ut.ee/demi/).

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