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RNA-Seq of GNRH1 neurons differentiated from human pluripotent stem cells (2)

ABSTRACT: This experiment was conducted to identify the transcriptome of human GNRH1 cells which has never been reported before and this experiment has identified some novel molecules which might regulate GNRH1 neuron differentiation which could be further explored to identify the origin and development of GNRH1 neurons in humans

INSTRUMENT(S): Sony SH800, NextSeq 500

ORGANISM(S): Homo sapiens

SUBMITTER: Venkatram Yellapragada

PROVIDER: E-MTAB-7956 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Development of Gonadotropin-Releasing Hormone-Secreting Neurons from Human Pluripotent Stem Cells.

Lund Carina C Pulli Kristiina K Yellapragada Venkatram V Giacobini Paolo P Lundin Karolina K Vuoristo Sanna S Tuuri Timo T Noisa Parinya P Raivio Taneli T

Stem cell reports 20160714 2

Gonadotropin-releasing hormone (GnRH) neurons regulate human puberty and reproduction. Modeling their development and function in vitro would be of interest for both basic research and clinical translation. Here, we report a three-step protocol to differentiate human pluripotent stem cells (hPSCs) into GnRH-secreting neurons. Firstly, hPSCs were differentiated to FOXG1, EMX2, and PAX6 expressing anterior neural progenitor cells (NPCs) by dual SMAD inhibition. Secondly, NPCs were treated for 10 d ...[more]

PMID: 27426041

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RNA-Seq of GNRH1 neurons differentiated from human pluripotent stem cells (1)

Project description:This experiment was conducted to identify the transcriptome of human GNRH1 cells which has never been reported before and this experiment has identified some novel molecules which might regulate GNRH1 neuron differentiation which could be further explored to identify the origin and development of GNRH1 neurons in humans

2020-02-16 | E-MTAB-8519 | biostudies-arrayexpress

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Gene expression of A53T and corrected IPSC driven to DA neurons and exposed to paraquat and maneb

Project description:Parkinson disease (PD) is characterized by extensive loss of A9 dopaminergic (DA) neurons in the substantia nigra pars compacta (SNpc). A strong association has been reported between PD and exposure to mitochondrial toxins such as the environmental pesticides paraquat, maneb, and rotenone. Here, using a robust, patient-derived, stem cell model of PD that allows comparison of -synuclein ( -syn) mutant cells and isogeneic mutation-corrected controls, we identify mitochondrial toxin-induced perturbations specific to A53T -syn mutant A9-DA neurons (hNs). We report a novel molecular pathway whereby basal as well as toxin-induced oxidative and nitrosative stress inhibits the MEF2C-PGC1 transcription network in A53T hNs compared to corrected controls, contributing to mitochondrial dysfunction and apoptotic cell death. Our data provide mechanistic insight into gene-environmental interaction (GxE) in the pathogenesis of PD. Furthermore, using small molecule high-throughput screening, we identify the MEF2C-PGC1 pathway as a new drug target for therapeutic benefit in PD. In the current study, isogenic hiPSCs differing exclusively at a single amino acid (A53T) were exposed to either 2.8uM paraquat in combination with 1uM maneb for 24h or PBS vehicle control. Gene expression profile was analysed to assess the effect of both the genotype and exposure regiment on gene expression.

2013-12-05 | E-GEOD-46798 | biostudies-arrayexpress

Nociceptor differentiation from pluripotent cells

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RNA-Seq profiling of dopaminergic neurons from iPSCs of Parkinson's disease patients

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2016-08-24 | E-MTAB-4868 | biostudies-arrayexpress

Distinct and shared functions of ALS-associated TDP-43, FUS, and TAF15 revealed by comprehensive multi-system integrative analyses [RNA-Seq_human]

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2016-07-05 | E-GEOD-77702 | biostudies-arrayexpress

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