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RNA-seq comparison of fibro-adipogenic progenitors (FAPs) from wild type, mdx and cardiotoxin-injured mice

ABSTRACT: We purified FAPs from the hind limbs of wild type, mdx and cardiotoxin-injured mice in order to unveil changes in their transcriptomes. RNA was isolated directly from sorted cells and analyzed by 3’ RNA sequencing.

INSTRUMENT(S): NextSeq 500

ORGANISM(S): Mus musculus

SUBMITTER: Gianni Cesareni

PROVIDER: E-MTAB-8040 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Fibro-adipogenic progenitors of dystrophic mice are insensitive to NOTCH regulation of adipogenesis.

Marinkovic Milica M Fuoco Claudia C Sacco Francesca F Cerquone Perpetuini Andrea A Giuliani Giulio G Micarelli Elisa E Pavlidou Theodora T Petrilli Lucia Lisa LL Reggio Alessio A Riccio Federica F Spada Filomena F Vumbaca Simone S Zuccotti Alessandro A Castagnoli Luisa L Mann Matthias M Gargioli Cesare C Cesareni Gianni G

Life science alliance 20190625 3

Fibro-adipogenic progenitors (FAPs) promote satellite cell differentiation in adult skeletal muscle regeneration. However, in pathological conditions, FAPs are responsible for fibrosis and fatty infiltrations. Here we show that the NOTCH pathway negatively modulates FAP differentiation both in vitro and in vivo. However, FAPs isolated from young dystrophin-deficient <i>mdx</i> mice are insensitive to this control mechanism. An unbiased mass spectrometry-based proteomic analysis of FAPs from musc ...[more]

PMID: 31239312

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