Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

0

DNA-seq of an ELMSeq reporter cassette to study impact of C-terminal sequence variations on protein abundance


ABSTRACT: A ELMSeq reporter cassette was created to monitor Dam levels by methylation, and introduced in the genome. The regions of 6 nt upstream and 6 nt downstream the stop codon were randomized to study their effect on gene expression. The ELMSeq reporter cassette was composed of: promoter - dam - random N6 - stop codon TAA - random N6 - spacer - 4xGATC. The amplicon was spanning the C-terminal region. The cassette was introduced in Mycoplasma pneumoniae.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mycoplasma pneumoniae

SUBMITTER: Marc Weber 

PROVIDER: E-MTAB-8223 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

Similar Datasets

2017-11-08 | E-MTAB-5365 | biostudies-arrayexpress
2018-03-22 | E-MTAB-5363 | biostudies-arrayexpress
2017-05-16 | E-MTAB-5673 | biostudies-arrayexpress
2024-04-05 | E-MTAB-13302 | biostudies-arrayexpress
2024-04-05 | E-MTAB-13258 | biostudies-arrayexpress
2023-06-01 | E-MTAB-12289 | biostudies-arrayexpress
2023-03-11 | PXD033854 | Pride
2022-10-25 | PXD034217 | Pride
2023-06-01 | E-MTAB-11786 | biostudies-arrayexpress
2021-10-27 | PXD025597 | Pride