Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Analysis of microRNA proflie of peripheral blood mononuclear cells (PBMCs) in HIV-1 infected patients under different clinical status respect to hepatitis C virus (HCV) infection.

ABSTRACT: A total of 117 HIV-1 infected patients were analyzed. These individuals were categorized in three different groups according to their infectious status for HCV: A) HIV+/HCV+ group, (n=45) patients with HIV infection and active HCV-chronic infection naïve to any HCV treatment (positive PCR and positive HCV antibodies); B) HIV+/HCV- group, (n=36) patients who had been exposed to HCV but experienced spontaneous viral clearance during the first 6 months after HCV infection (negative PCR and positive HCV antibodies); C) HIV+ group, (n=36) HIV+ mono-infected patients that had never been infected with HCV (negative PCR, negative HCV antibody and no previous HCV treatment). Briefely, peripheral venous blood samples were collected in EDTA tubes, and PBMCs were isolated within the first 4 hours after extraction.Total RNA including miRNAs were isolated from PBMCs with the miRNeasy Mini kit (Qiagen). Quality and integrity were evaluated by the Bioanalyzer 2100 with Agilent RNA 6000 Nano kit (Agilent). Only those samples with RIN > 7.5 were sequenced. Small RNA library synthesis and sequencing were performed at Centre for Genomic Regulation (CRG) at Barcelona (Spain). Small RNA libraries were constructed with Illumina’s TruSeq Small RNA kit v.4 (Illumina) and 50nts (1x50) were sequenced in an Illumina HiSeq2500, with a single read approach.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Homo sapiens

SUBMITTER: Amanda Fernández Rodríguez

PROVIDER: E-MTAB-8274 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

HCV eradication with DAAs differently affects HIV males and females: A whole miRNA sequencing characterization.

Valle-Millares Daniel D Brochado-Kith Óscar Ó Gómez-Sanz Alicia A Martín-Carbonero Luz L Ryan Pablo P De Los Santos Ignacio I Castro Juan M JM Troya Jesús J Mayoral-Muñoz Mario M Cuevas Guillermo G Martínez-Román Paula P Sanz-Sanz Jesús J Muñoz-Muñoz María M Jiménez-Sousa María Á MÁ Resino Salvador S Briz Verónica V Fernández-Rodríguez Amanda A

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 20211112

Gender-specific consequences after HCV eradication are unexplored. MicroRNAs (miRNAs) play a crucial role in the immune response against viral infections. However, few have highlighted miRNA role in sex-biased disease or therapy response. We aim to assess gender differences reflected in the miRNA expression of HIV/HCV-coinfected patients who achieve sustained virological response (SVR) with direct acting antivirals (DAAs). We conducted a prospective study of miRNA expression in PBMCs from 28 chr ...[more]

PMID: 34781145

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Project description:Hepatitis C virus (HCV) remains a significant public health threat as new 1.75 million HCV infections emerged worldwide. The majority of these infections become persistently infected, while around 30 % spontaneously eliminate the virus. Clinical factors for viral clarification are related to HCV interaction with host immune system, but little is known about the consequences after HCV spontaneous resolution. These individuals are difficult to recruit and study as acute infection is usually asymptomatic, and they will not be identified unless it progress to chronic infection. The study of peripheral blood mononuclear cells (PBMCs) of these patients is crucial, as PBMCs are one of the main HCV extrahepatic reservoirs, and its transcriptional profile provide us information of innate and adaptive immune response against HCV infection. Our research shows novel insight on molecular consequences of spontaneous resolution after an acute HCV infection. 96 Individuals with different HCV exposure status were recruited: spontaneous resolved, chronic infected and healthy controls; and the microRNA profile of their PBMCs were analyzed. Our results indicate similar disruption of miRNA expression on HCV chronic patients and those who spontaneously clarified the infection, compared to control patients. The disrupted miRNAs formed a signature of 21 miRNAs that mainly regulate lipid metabolism. This is the first report showing miRNA profile similarities between chronic HCV patients and spontaneous resolved individuals. Thus, our results suggest that HCV infection promotes molecular alterations in PBMCs that will last longer after HCV spontaneous eradication. This evidences open up new prospects in the management of individuals who spontaneously clarified infection, as they should be monitored and followed to dismiss future HCV-related complications, such us liver diseases complications. The identified miRNA signature could be used as biomarker to monitor HCV fingerprint on HCV-exposed patients.

Dataset Information

Analysis of microRNA proflie of peripheral blood mononuclear cells (PBMCs) in HIV-1 infected patients under different clinical status respect to hepatitis C virus (HCV) infection.

Publications

HCV eradication with DAAs differently affects HIV males and females: A whole miRNA sequencing characterization.

Similar Datasets

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