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Examination of mRNA decay rate in mature adipocytes

ABSTRACT: The CCR4-NOT deadenylase complex has a critical function to trigger mRNA decay and various biological events. To understand roles of the complex in adipocyte function, we compare mRNA half-lives between control and Cnot complex-deficient mature adipocytes from iWAT and BAT.

INSTRUMENT(S): Illumina HiSeq 2500

ORGANISM(S): Mus musculus

SUBMITTER: Toru Suzuki

PROVIDER: E-MTAB-8371 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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The CCR4-NOT Deadenylase Complex Maintains Adipocyte Identity.

Takahashi Akinori A Takaoka Shohei S Kobori Shungo S Yamaguchi Tomokazu T Ferwati Sara S Kuba Keiji K Yamamoto Tadashi T Suzuki Toru T

International journal of molecular sciences 20191024 21

Shortening of poly(A) tails triggers mRNA degradation; hence, mRNA deadenylation regulates many biological events. In the present study, we generated mice lacking the Cnot1 gene, which encodes an essential scaffold subunit of the CCR4-NOT deadenylase complex in adipose tissues (Cnot1-AKO mice) and we examined the role of CCR4-NOT in adipocyte function. Cnot1-AKO mice showed reduced masses of white adipose tissue (WAT) and brown adipose tissue (BAT), indicating abnormal organ ...[more]

PMID: 31652943

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Expression data from mouse embryonic fibroblasts

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Human Pumilio proteins directly bind the CCR4-NOT deadenylase complex to regulate the transcriptome

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