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Examination of mRNA decay rate in mouse embryonic fibroblasts

ABSTRACT: The CCR4-NOT dedadenylase complex is essential for mRNA decay and various biological events.To understand roles of the complex in mouse embryonic fibroblasts, we compared mRNA half-lives between control and Cnot complex-deficient mouse embryonic fibroblasts

INSTRUMENT(S): Illumina HiSeq 4000

ORGANISM(S): Mus musculus

SUBMITTER: Dina Mostafa

PROVIDER: E-MTAB-8287 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Essential functions of the CNOT7/8 catalytic subunits of the CCR4-NOT complex in mRNA regulation and cell viability.

Mostafa Dina D Takahashi Akinori A Yanagiya Akiko A Yamaguchi Tomokazu T Abe Takaya T Kureha Taku T Kuba Keiji K Kanegae Yumi Y Furuta Yasuhide Y Yamamoto Tadashi T Suzuki Toru T

RNA biology 20200110 3

Shortening of mRNA poly(A) tails (deadenylation) to trigger their decay is mediated mainly by the CCR4-NOT deadenylase complex. While four catalytic subunits (CNOT6, 6L 7, and 8) have been identified in the mammalian CCR4-NOT complex, their individual biological roles are not fully understood. In this study, we addressed the contribution of CNOT7/8 to viability of primary mouse embryonic fibroblasts (MEFs). We found that MEFs lacking CNOT7/8 expression [<i>Cnot7/8</i>-double knockout (dKO) MEFs] ...[more]

PMID: 31924127

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