Cross-linking ligation and sequencing of hybrids (qCLASH) reveals an unpredicted miRNA targetome in melanoma cells
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ABSTRACT: In the quick cross-linking ligation and sequencing of hybrids (qCLASH) protocol, RNA-protein (RNP) complexes of interest are UV cross-linked in living cells. The protein of interest is purified by immunoprecipitation (in this case, AGO bound to the miRNA and the target gene). The two interacting RNA molecules (e.g. miRNA-mRNA) are physically bound to each other by intermolecular RNA-RNA ligation, followed by library preparation and sequencing oh hybrids.
INSTRUMENT(S): Illumina HiSeq 3000
ORGANISM(S): Homo sapiens
SUBMITTER: Ines Kozar
PROVIDER: E-MTAB-8591 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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