Unknown,Transcriptomics,Genomics,Proteomics

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Small RNA from S2 cell infected with FHV deltaB2 particle


ABSTRACT: S2 cells were infected with FHV deltaB2 particle and the S2 cells were harvested to isolate total RNA at 96 hpi. Small RNAs were separated on a denaturing 15% polyacrylamide gel. Ligation to adapters requires 5' monophosphate and 3' OH. Note: The base quality (.qual) file corresponding to the fasta format (.fna) file linked to Sample GSM306489 is unavailable. Small RNAs were sequenced from S2 cell after virus infection.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Qingfa Wu 

PROVIDER: E-GEOD-12527 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Mechanism of induction and suppression of antiviral immunity directed by virus-derived small RNAs in Drosophila.

Aliyari Roghiyh R   Wu Qingfa Q   Li Hong-Wei HW   Wang Xiao-Hong XH   Li Feng F   Green Lance D LD   Han Cliff S CS   Li Wan-Xiang WX   Ding Shou-Wei SW  

Cell host & microbe 20081001 4


The small RNA-directed viral immunity pathway in plants and invertebrates begins with the production by Dicer nuclease of virus-derived siRNAs (viRNAs), which guide specific antiviral silencing by Argonaute protein in an RNA-induced silencing complex (RISC). Molecular identity of the viral RNA precursor of viRNAs remains a matter of debate. Using Flock house virus (FHV) infection of Drosophila as a model, we show that replication of FHV positive-strand RNA genome produces an approximately 400 bp  ...[more]

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