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HeLa mRNA interactome

ABSTRACT: Pull-down of poly(A)-mRNA cross linked proteins using two cross-linking methods (conventional cross-linking and PAR-cross-linking) to identify all mRNA-binding proteins (GO:0003729). The provided data is quantitative proteomic data for comparison of cross-linking and control samples.

ORGANISM(S): Homo sapiens

SUBMITTER: Bernd Fischer

PROVIDER: E-MTAB-869 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Insights into RNA biology from an atlas of mammalian mRNA-binding proteins.

Castello Alfredo A Fischer Bernd B Eichelbaum Katrin K Horos Rastislav R Beckmann Benedikt M BM Strein Claudia C Davey Norman E NE Humphreys David T DT Preiss Thomas T Steinmetz Lars M LM Krijgsveld Jeroen J Hentze Matthias W MW

Cell 20120531 6

RNA-binding proteins (RBPs) determine RNA fate from synthesis to decay. Employing two complementary protocols for covalent UV crosslinking of RBPs to RNA, we describe a systematic, unbiased, and comprehensive approach, termed "interactome capture," to define the mRNA interactome of proliferating human HeLa cells. We identify 860 proteins that qualify as RBPs by biochemical and statistical criteria, adding more than 300 RBPs to those previously known and shedding light on RBPs in disease, RNA-bin ...[more]

PMID: 22658674

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