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MicroRNA profiling using microarrays of two human metformin-treated non-small cell lung cancer cell-lines

ABSTRACT: To examine the effect of metformin on lung cancer biology, human lung H226 and H1299 squamous cell carcinoma cell-lines were grown in RPMI-1640 medium with 10% v/v fetal bovine serum and with or without 15 uM metformin hydrochloride for 7-8 days. Medium (with any metformin) was replaced every two days. Paired cultures with and without metformin were grown and maintained in parallel. Three separate paired cultures, all seeded with same stock of frozen cells, were grown.

ORGANISM(S): Homo sapiens

SUBMITTER: Santosh Patnaik

PROVIDER: E-MTAB-8782 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcriptome profiling using microarrays of four human metformin-treated non-small cell lung cancer cell-lines

Project description:To examine the effect of metformin on lung cancer biology, human lung A549 adenocarcinoma, H460 large cell carcinoma, and H226 and H1299 squamous cell carcinoma cell-lines were grown in RPMI-1640 medium with 10% v/v fetal bovine serum and with or without 15 uM metformin hydrochloride for 7-8 days. Medium (with any metformin) was replaced every two days. Paired cultures with and without metformin were grown and maintained in parallel. Three separate paired cultures, all seeded with same stock of frozen cells, were grown. Cells, within 15%-95% confluence range, were harvested by scraping. Total RNA from cells was prepared using Norgen Biotek® Total RNA Isolation kit (with on-column DNAse I treatment). All RNA integrity number (RIN) values were greater than 8.2.

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Differential Expression of microRNAs following activated protein C treatment in a rat model of septic shock

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Expression data of human fetal liver hematopoietic stem and progenitors cells [Set 2]

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2015-01-23 | E-GEOD-54315 | biostudies-arrayexpress

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2015-01-23 | E-GEOD-54314 | biostudies-arrayexpress

Microarray analysis of human squamous cell lung cancer-H226 cells transfected with DDX56 siRNA and control siRNA

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2019-07-01 | E-MTAB-6675 | biostudies-arrayexpress

Expression data from aortic smooth muscle cells (AoSMC) and myofibroblasts (FB) isolated respectively from the ascending aortas and the aortic valves of bicuspid (BAV) and tricuspid aortic valve (TAV) patients

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2016-01-04 | E-GEOD-76250 | biostudies-arrayexpress

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