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Pancreas-specific deletion of Ikba and RelA.

ABSTRACT: The NfkB-pathway is activated early during acute pancreatitis. We investigated the influence on gene expression of two pancreas-specific deletions interfering with NfkB-activation. Pancreata from 8 week old mice were prepared, RNA was isolated and Affymetrix microarray expression analysis was performed.

ORGANISM(S): Mus musculus

SUBMITTER: Henrik Einwaechter

PROVIDER: E-MTAB-886 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Deletion of IκBα activates RelA to reduce acute pancreatitis in mice through up-regulation of Spi2A.

Neuhöfer Patrick P Liang Song S Einwächter Henrik H Schwerdtfeger Christiane C Wartmann Thomas T Treiber Matthias M Zhang Hong H Schulz Hans-Ulrich HU Dlubatz Karen K Lesina Marina M Diakopoulos Kalliope N KN Wörmann Sonja S Halangk Walter W Witt Heiko H Schmid Roland M RM Algül Hana H

Gastroenterology 20121003 1

<h4>Background & aims</h4>The transcription factor nuclear factor-κB (NF-κB) (a heterodimer of NF-κB1p50 and RelA) is activated rapidly in acute pancreatitis (AP). However, it is not clear whether NF-κB promotes or protects against AP. We used the NF-κB inhibitor protein, inhibitor of κB (IκB)α, to study the roles of NF-κB in the development of AP in mice.<h4>Methods</h4>IκBα or the combination of IκBα and RelA selectively were deleted from pancreas of mice using the Cre/locus of cross-over P st ...[more]

PMID: 23041330

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