Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

DNA methylation signature mirrors successful weight-loss during lifestyle interventions in blood

ABSTRACT: DNA methylation analysis using HumanMethylation850K BeadChips (Illumina) in human blood samples before and after an 18 months weight-loss intervention: the CENTRAL study [randomized controlled trial; NCT01530724]. All subjects underwent either Mediterranean/low-carbohydrate or low-fat diet with or without physical activity.

ORGANISM(S): Homo sapiens

SUBMITTER: Maria Keller

PROVIDER: E-MTAB-8956 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Effect of Distinct Lifestyle Interventions on Mobilization of Fat Storage Pools: CENTRAL Magnetic Resonance Imaging Randomized Controlled Trial.

Gepner Yftach Y Shelef Ilan I Schwarzfuchs Dan D Zelicha Hila H Tene Lilac L Yaskolka Meir Anat A Tsaban Gal G Cohen Noa N Bril Nitzan N Rein Michal M Serfaty Dana D Kenigsbuch Shira S Komy Oded O Wolak Arik A Chassidim Yoash Y Golan Rachel R Avni-Hassid Hila H Bilitzky Avital A Sarusi Benjamin B Goshen Eyal E Shemesh Elad E Henkin Yaakov Y Stumvoll Michael M Blüher Matthias M Thiery Joachim J Ceglarek Uta U Rudich Assaf A Stampfer Meir J MJ Shai Iris I

Circulation 20171115 11

<h4>Background</h4>We aimed to assess whether distinct lifestyle strategies can differentially affect specific body adipose depots.<h4>Methods</h4>We performed an 18-month randomized controlled trial among 278 sedentary adults with abdominal obesity (75%) or dyslipidemia in an isolated workplace with a monitored provided lunch. Participants were randomized to isocaloric low-fat or Mediterranean/low-carbohydrate (MED/LC) diet+28 g walnuts/day with/without added moderate physical activity (PA; 80% ...[more]

PMID: 29142011

Publication: 1/2

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Project description:A fundamental challenge in the post-genome era is to understand and annotate the consequences of genetic variation, particularly within the context of human tissues. We describe a set of integrated experiments designed to investigate the effects of common genetic variability on DNA methylation, mRNA expression and microRNA (miRNA) expression in four distinct human brain regions. We show that brain tissues may be readily distinguished based on methylation status or expression profile. We find an abundance of genetic cis regulation mRNA expression and show for the first time abundant quantitative trait loci for DNA CpG methylation. We observe that the largest magnitude effects occur across distinct brain regions. We believe these data, which we have made publicly available, will be useful in understanding the biological effects of genetic variation. Authorized Access data: Mapping of GEO sample accessions to dbGaP subject/sample IDs is available through dbGaP Authorized Access, see http://www.ncbi.nlm.nih.gov/projects/gap/cgi-bin/study.cgi?study_id=phs000249.v1.p1 Because of our interest in genomic regulation of expression and neurological disorders we embarked upon a series of experiments to provide a brain region-specific contextual framework for genetic and epigenetic regulation of gene expression. We obtained frozen brain tissue from the cerebellum, frontal cortex, pons and temporal cortex from 150 subjects (total 600 tissue samples). We undertook four separate assays across this series; first, genome-wide SNP genotyping; second, assay of >27,000 CpG methylation sites in each of the four brain regions; third, mRNA expression profiling of >22,000 transcripts in all four brain regions; and, fourth, miRNA expression profiling of 735 miRNA transcripts. Here we discuss the results of these experiments, particularly in the context of integrated datasets to define expression and CpG methylation quantitative trait loci (eQTL and methQTL) and detailing differences and similarities across brain regions.

Dataset Information

DNA methylation signature mirrors successful weight-loss during lifestyle interventions in blood

Publications

Effect of Distinct Lifestyle Interventions on Mobilization of Fat Storage Pools: CENTRAL Magnetic Resonance Imaging Randomized Controlled Trial.

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