Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

Transcription profiling of mouse E10.5 female embryos wild-type and mutant for Smchd1

ABSTRACT: To investigate the role of the Smchd1 protein in epigenetic silencing of autosomal loci, we carried out a comparative microarray gene expression analysis of wild-type and Smchd1-/- female embryos at E10.5. Embryos were collected at E10.5 after natural mating, sexed and genotyped. Total RNAs were extracted from three individual wild-type and three Smchd1-/- female embryos and cRNAs prepared from these samples was hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 array.

ORGANISM(S): Mus musculus

SUBMITTER: Anne-Valerie Gendrel

PROVIDER: E-MTAB-921 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Epigenetic functions of smchd1 repress gene clusters on the inactive X chromosome and on autosomes.

Gendrel Anne-Valerie AV Tang Y Amy YA Suzuki Masako M Godwin Jonathan J Nesterova Tatyana B TB Greally John M JM Heard Edith E Brockdorff Neil N

Molecular and cellular biology 20130610 16

The Smchd1 gene encodes a large protein with homology to the SMC family of proteins involved in chromosome condensation and cohesion. Previous studies have found that Smchd1 has an important role in CpG island (CGI) methylation on the inactive X chromosome (Xi) and in stable silencing of some Xi genes. In this study, using genome-wide expression analysis, we showed that Smchd1 is required for the silencing of around 10% of the genes on Xi, apparently independent of CGI hypomethylation, and, more ...[more]

PMID: 23754746

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Dataset Information

Transcription profiling of mouse E10.5 female embryos wild-type and mutant for Smchd1

Publications

Epigenetic functions of smchd1 repress gene clusters on the inactive X chromosome and on autosomes.

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