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Gene regulation by CBP-p300 in the lens placode - E10.5

ABSTRACT: Wild type or CBP/p300 fx/fx; Pax6-Cre-positive (CBP/p300-/-) surface ectoderm from E10.5 mouse embryos was laser micodissected from three embryos of each genotype. Total RNA was purified, pooled for each genotype and triplicate samples were reverse transcribed and amplified using a NuGEN kit. cDNA was biotinylated and hybridized to Illumina Mouse6v2.0 bead arrays. Three wild type and three knockout embryos were used. The RNA from embryos of the same genotype was purified and pooled and triplicate samples were amplified and used for microarray analysis.

ORGANISM(S): Mus musculus

SUBMITTER: David Beebe 

PROVIDER: E-GEOD-49218 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Histone posttranslational modifications and cell fate determination: lens induction requires the lysine acetyltransferases CBP and p300.

Wolf Louise L   Harrison Wilbur W   Huang Jie J   Xie Qing Q   Xiao Ningna N   Sun Jian J   Kong Lingkun L   Lachke Salil A SA   Kuracha Murali R MR   Govindarajan Venkatesh V   Brindle Paul K PK   Ashery-Padan Ruth R   Beebe David C DC   Overbeek Paul A PA   Cvekl Ales A  

Nucleic acids research 20130912 22

Lens induction is a classical embryologic model to study cell fate determination. It has been proposed earlier that specific changes in core histone modifications accompany the process of cell fate specification and determination. The lysine acetyltransferases CBP and p300 function as principal enzymes that modify core histones to facilitate specific gene expression. Herein, we performed conditional inactivation of both CBP and p300 in the ectodermal cells that give rise to the lens placode. Ina  ...[more]

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