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ClariomS Array to determine the effect of a vaspin-derived peptide on gene expression in fully differentiated primary subcutaneous mouse adipocytes

ABSTRACT: Screening for potential target proteases of vaspin revealed rapid and specific cleavage within the vaspin N-terminus, releasing short peptides with cell penetrating activity. For microarray analysis to determine the effect of a vaspin-derived peptide on gene expression in adipocytes, fully differentiated primary mouse adipocytes from subcutaneous AT were treated with 100 nM of peptide for 24 h before harvesting, RNA isolation and microarray analysis. Solvent (water) treated cells served as controls.

ORGANISM(S): Mus musculus

SUBMITTER: Anne Hoffmann

PROVIDER: E-MTAB-9592 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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