MRNA profiling of NMuMG mouse mammary epithelial cells incubated for 4 weeks in the presence of aluminium chloride vs controls incubated in parallel in the presence of the same dilution of solvent (H2O) alone

ABSTRACT: Salts of aluminium, a chemical element devoid of biological function, are added to many industrial products of frequent use because of aluminium's chemical versatility. As a result of lifetime exposure, aluminium accumulates in several organs including the mammary gland. At concentrations in the range of those measured in the breast of women living in industrialised countries, aluminium chloride transforms human and mouse mammary epithelial cells in vitro. These results challenge the safety commonly ascribed to aluminium and suggest that it could be a human carcinogen. NMuMG is a spontaneously immortalised, non tumorigenic mouse mammary epithelial cell line originally derived from NAMRU mice that, following chronic culture in the presence of aluminium - in the form of AlCl3 x 6H2O - becomes able to form tumors and metastasis in NOD-scid and nude mice. As a part of our investigations on the mechanisms by which aluminium might transform mammary epithelial cells, we analysed the mRNA profile of NMuMG cells incubated for 4 weeks - a time duration that precedes cellular transformation - in the presence of aluminium or the corresponding untreated controls cultured in parallel, by cDNA microarray. We used the Clariom S microarray from Thermofisher, that covers more than 20'000 well annotated mRNAs.

ORGANISM(S): Mus musculus

SUBMITTER: Stefano Mandriota 

PROVIDER: E-MTAB-9764 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress