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Genome-wide Analysis of Sp2 Occupancy and Expression Profiling Establish Sp2 as a Sequence-specific Transcription Factor Regulating Fundamental Cellular Processes - ChIPseq

ABSTRACT: Genome-wide Analysis of Sp2 Occupancy and Expression Profiling Establish Sp2 as a Sequence-specific Transcription Factor Regulating Fundamental Cellular Processes - ChIPseq

ORGANISM(S): Homo sapiens

SUBMITTER: Florian Finkernagel

PROVIDER: E-MTAB-994 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Genome-wide localization and expression profiling establish Sp2 as a sequence-specific transcription factor regulating vitally important genes.

Terrados Gloria G Finkernagel Florian F Stielow Bastian B Sadic Dennis D Neubert Juliane J Herdt Olga O Krause Michael M Scharfe Maren M Jarek Michael M Suske Guntram G

Nucleic acids research 20120607 16

The transcription factor Sp2 is essential for early mouse development and for proliferation of mouse embryonic fibroblasts in culture. Yet its mechanisms of action and its target genes are largely unknown. In this study, we have combined RNA interference, in vitro DNA binding, chromatin immunoprecipitation sequencing and global gene-expression profiling to investigate the role of Sp2 for cellular functions, to define target sites and to identify genes regulated by Sp2. We show that Sp2 is import ...[more]

PMID: 22684502

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