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Transcription profiling of wild type, pol-beta knock-out and pol-beta-knockout plus pol-beta mouse embryonic fibroblasts from confluent and log phase cultures after ionizing radiation treatment

ABSTRACT: wt, polbeta ko, ko+polbeta log versus confluent ionizing radiation (exp1)

ORGANISM(S): Mus musculus

SUBMITTER: Cor Lieftink

PROVIDER: E-NCMF-10 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Transcription profiling of human breast carcinomas negative for estrogen, progesterone and HER2/nue receptors

Project description:97 triple negative tumors were selected from the fresh-frozen tissue bank of the Netherlands Cancer Institute and gene expression profiles were generated using 35K oligonucleotide microarrays. Human breast carcinomas were snap frozen in liquid nitrogen within one hour after surgery and stored in the fresh-frozen tissue bank of the Netherlands Cancer Institute. RNA from a pool of more than 100 unselected fresh frozen breast carcinomas were isolated and pooled to form the reference to which each individual breast carcinoma is hybridized.

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