Ontology highlight
ABSTRACT:
ORGANISM(S): Homo sapiens
SUBMITTER: Janos Demeter
PROVIDER: E-SMDB-1609 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
BMC genomics 20021030 1
<h4>Background</h4>T7 based linear amplification of RNA is used to obtain sufficient antisense RNA for microarray expression profiling. We optimized and systematically evaluated the fidelity and reproducibility of different amplification protocols using total RNA obtained from primary human breast carcinomas and high-density cDNA microarrays.<h4>Results</h4>Using an optimized protocol, the average correlation coefficient of gene expression of 11,123 cDNA clones between amplified and unamplified ...[more]