Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

Dataset Information

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Transcription profiling of glucose limited yeast, chemostat batch growth, time series 1


ABSTRACT: Time course of batch growth. Reference (channel 1) was a culture grown in MD medium with 2.4 g/L glucose, with 5 slpm air-flow, stirring at 400 rpm and a constant 300C temperature, dilution 0.25 volumes/hour. The experimental (channel 2) time course samples were grown in batch for the indicated time. The "Low-D chemostat vs. High-D chemostat" hybridization's channel 2 sample was grown in a chemostat, with a dilution rate of 0.05 volumes/hour; it is most similar to the time course samples taken between 8 and 9 hours.

ORGANISM(S): Saccharomyces cerevisiae

SUBMITTER: Tina Boussard 

PROVIDER: E-SMDB-3252 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Publications

Homeostatic adjustment and metabolic remodeling in glucose-limited yeast cultures.

Brauer Matthew J MJ   Saldanha Alok J AJ   Dolinski Kara K   Botstein David D  

Molecular biology of the cell 20050309 5


We studied the physiological response to glucose limitation in batch and steady-state (chemostat) cultures of Saccharomyces cerevisiae by following global patterns of gene expression. Glucose-limited batch cultures of yeast go through two sequential exponential growth phases, beginning with a largely fermentative phase, followed by an essentially completely aerobic use of residual glucose and evolved ethanol. Judging from the patterns of gene expression, the state of the cells growing at steady  ...[more]

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