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Metabolomics,Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling of human fibroblast cells from either basal cell carcinoma or non-tumor skin tissue

ABSTRACT: Fibroblast cells were cultured from human skin, either tumor (basal cell carcinoma) or non-tumor tissue. Cells were propagated for several passages in culture, then switched to low (0.1%) serum conditions for 48 hours prior to mRNA harvest. Reference for all arrays was Stratagene Universal Human Reference RNA.

ORGANISM(S): Homo sapiens

SUBMITTER: Janos Demeter

PROVIDER: E-SMDB-3838 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Bone morphogenetic protein antagonist gremlin 1 is widely expressed by cancer-associated stromal cells and can promote tumor cell proliferation.

Sneddon Julie B JB Zhen Hanson H HH Montgomery Kelli K van de Rijn Matt M Tward Aaron D AD West Robert R Gladstone Hayes H Chang Howard Y HY Morganroth Greg S GS Oro Anthony E AE Brown Patrick O PO

Proceedings of the National Academy of Sciences of the United States of America 20060926 40

Although tissue microenvironments play critical roles in epithelial development and tumorigenesis, the factors mediating these effects are poorly understood. In this work, we used a genomic approach to identify factors produced by cells in the microenvironment of basal cell carcinoma (BCC) of the skin, one of the most common human cancers. The global gene expression programs of stromal cell cultures derived from human BCCs showed consistent, systematic differences from those derived from nontumo ...[more]

PMID: 17003113

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