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Comparative genomic hybridization of 17 favourable histology Wilms tumour samples taken at immediate nephrectomy in order to identify changes in DNA copy number associated with clinical outcome

ABSTRACT: We have carried out microarray-based comparative genomic hybridisation (arrayCGH) on 17 Favourable histology Wilms tumour samples taken at immediate nephrectomy in order to identify changes in DNA copy number associated with clinical outcome. Tumour DNA was co-hybridised with normal female genomic DNA onto microarrays consisting of 2118 BAC clones with tiling-path coverage of chromosome 1. Data was normalised and quality-filtered, an adapted weights smoothing algorithm fitted, and changes in DNA copy number assessed for each clone.

ORGANISM(S): Homo sapiens

DISEASE(S): Wilms tumour

SUBMITTER: Anita Grigoriadis

PROVIDER: E-TABM-13 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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Array CGH profiling of favourable histology Wilms tumours reveals novel gains and losses associated with relapse.

Natrajan R R Williams R D RD Hing S N SN Mackay A A Reis-Filho J S JS Fenwick K K Iravani M M Valgeirsson H H Grigoriadis A A Langford C F CF Dovey O O Gregory S G SG Weber B L BL Ashworth A A Grundy P E PE Pritchard-Jones K K Jones C C

The Journal of pathology 20060901 1

Despite the excellent survival of Wilms tumour patients treated with multimodality therapy, approximately 15% will suffer from tumour relapse, where response rates are markedly reduced. We have carried out microarray-based comparative genomic hybridisation on a series of 76 Wilms tumour samples, enriched for cases which recurred, to identify changes in DNA copy number associated with clinical outcome. Using 1Mb-spaced genome-wide BAC arrays, the most significantly different genomic changes betwe ...[more]

PMID: 16823893

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