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Unknown,Transcriptomics,Genomics,Proteomics

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Comparative genomic hybridization of plasmids containing genomic library clones grown in E. coli to monitor copy number changes over time

ABSTRACT: We report a genome-wide, multi-scale approach to simultaneously measure the effect that the increased copy of each gene and/or operon has on a desired trait or phenotype. The method involves i) growth selections on a mixture of several different plasmid based genomic libraries of defined insert sizes or SCALEs, ii) micro-array studies of enriched plasmid DNA, and a ii) mathematical multi-scale analysis (MSA) that precisely identifies the relevant genetic elements. This approach allows for identification of all single open reading frames and larger multi-gene fragments within a genomic library that alter the expression of a given phenotype. We have demonstrated this method in E. coli by monitoring, in parallel, a population of >106 genomic library clones of different insert sizes, throughout continuous selections over a period of 100 generations.

ORGANISM(S): Escherichia coli

SUBMITTER: Ryan Gill

PROVIDER: E-TABM-143 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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