Screening in E. coli overexpresssion libraries for genes protecting from histone killing
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ABSTRACT: The experiment's aim is to follow the population of E. coli cells before and after treatment with histones. The E. coli libraries consisted of MACH1 pSMART-LCKm carrying random fragments of 1, 2, 4 and 8 kb of E. coli genome. The selection procedure involved three rounds of histones treatment (same dose each time during 3h hours then recovery time of 2h before applying the next histone treatment, at the end a total of 15-16h).
ORGANISM(S): Escherichia coli
SUBMITTER: Catherine Chaput
PROVIDER: E-MEXP-3919 | biostudies-arrayexpress |
REPOSITORIES: biostudies-arrayexpress
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