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Transcription profiling of D.melanogaster twist homozygous mutants vs wildtype embryos at developmental stages

ABSTRACT: Expression profiling of Drosophila twist homozygous mutant embryos in a timecourse of embryogenesis. Four one-hour timepoints were assayed (4-8 hrs after egg laying). Homozygous mutant embryos were collected using GFP-activated embryo sorting at the same time as stage-matched wildtype controls. Four independent collections were performed at each timepoint. Total RNA was extracted and amplified. Mutant and respective wildtype samples were hybridized together to INDAC oligonucleotide arrays.

ORGANISM(S): Drosophila melanogaster

SUBMITTER: Charles Girardot

PROVIDER: E-TABM-161 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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A core transcriptional network for early mesoderm development in Drosophila melanogaster.

Sandmann Thomas T Girardot Charles C Brehme Marc M Tongprasit Waraporn W Stolc Viktor V Furlong Eileen E M EE

Genes & development 20070201 4

Embryogenesis is controlled by large gene-regulatory networks, which generate spatially and temporally refined patterns of gene expression. Here, we report the characteristics of the regulatory network orchestrating early mesodermal development in the fruitfly Drosophila, where the transcription factor Twist is both necessary and sufficient to drive development. Through the integration of chromatin immunoprecipitation followed by microarray analysis (ChIP-on-chip) experiments during discrete tim ...[more]

PMID: 17322403

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