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Unknown,Transcriptomics,Genomics,Proteomics

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Transcription profiling time series of rep1delta mutant fission yeast during meiosis

ABSTRACT: Changes in gene expression are hallmarks of cellular differentiation. Sexual differentiation in fission yeast (Schizosaccharomyces pombe) provides a model system for gene expression programs accompanying and driving cellular specialization. The expression of hundreds of genes is modulated in successive waves during meiosis and sporulation in S. pombe, and several known transcription factors are critical for these processes. We used DNA microarrays to investigate meiotic gene regulation by examining transcriptomes after genetic perturbations (gene deletion and/or overexpression) of rep1, mei4, atf21 and atf31, which encode known transcription factors controlling sexual differentiation. This analysis reveals target genes at a genome-wide scale and uncovers combinatorial control by Atf21p and Atf31p. We also studied two transcription factors that are upregulated during meiosis but had not been previously implicated in sexual differentiation : Rsv2p induces stress-related genes during spore formation, while Rsv1p acts as a repressor for glucose-metabolism genes. Our data further reveal negative feedback interactions: both Rep1p and Mei4p not only activate specific gene expression waves (early and middle genes, respectively), but are also required for repression of genes induced in the previous waves (Ste11p-dependent and early genes, respectively).

ORGANISM(S): Schizosaccharomyces pombe

SUBMITTER: Juan Mata

PROVIDER: E-TABM-300 | biostudies-arrayexpress |

REPOSITORIES: biostudies-arrayexpress

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